Buchwald Cross-Coupling HCK1004-01-002

2 sets of 15 reagent combinations premixed in same vial; 5 Catalysts (BrettPhosG2, RuPhosG2, XPhosG2, tBuXPhosG3 and tBuBrettG3) and 3 bases (K₂CO₃, K₃PO₄, and Cs₂CO₃). 30 reaction vials.

This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)

Protocol at 100 μl volume reaction condition

• Prepare required volume of a 0.15 mol/L solution of combined substrates in the desired sparged solvent. For example, 1600 μl solution for 15 reaction conditions (100 μl extra to compensate potential evaporation).
• Using a clean and dry syringe, add 100 μl of substrates solution to each reaction vial.
• Stir the reaction vials in the reaction block at desired temperature (for example: 80°C) for 3 to 12 hours.
• Remove the vial caps using a decapper.
• Prepare analytical sample for each reaction condition with 5 μl sample diluted into 200 μl in either DMSO or water/acetonitrile 50/50.
• Analyze resulting analytical samples by LC/MS.