Buchwald Cross-Coupling HCK1004-01-009

Buchwald Cross Coupling Array:
Pd Amination Weak Base Kit 6

2 sets of 32 reagent combinations premixed in same vial; 8 Catalysts (DavePhosG3, JackiePhosG3, JosiphosG3, P(t-Bu)3G4, PCy3G4, RockPhosG3, SPhosG3 and XantphosG3) and 4 bases (K2CO3, K3PO4, Cs2CO3, and KOAc) 64 reaction vials total.

This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)

The typical protocol is performed at 0.15 mol/l concentration reaction condition and 1 to 1 ratio substrates in 100 μl selected solvent. Resulting catalyst loading is 10%.Two equivalents of base are preloaded (30 μmol). It is possible to decrease substrate concentration by increasing the reaction volume (see chart below). Maximum volume for reaction condition is 250 μl. Recommended solvents are tBuOH or Toluene.

Protocol at 100 μl volume reaction condition

  • Prepare required volume of a 0.15 mol/L solution of combined substrates in the desired sparged solvent. For example, 3300 μl solution for 32 reaction conditions (100 μl extra to compensate potential evaporation).
  • Using a clean and dry syringe, add 100 μl of substrates solution to each reaction vial.
  • Stir the reaction vials in the reaction block at desired temperature (for example: 80°C) for 3 to 12 hours.
  • Remove the vial caps using a decapper.
  • Prepare analytical sample for each reaction condition with 5 μl sample diluted into 200 μl in either DMSO or water/acetonitrile 50/50.

Analyze resulting analytical samples by LC/MS.