Amide Coupling Array:
Phosphonium Coupling Reagent Kit 2
(HCK1005-01-006)
2 sets of 7 reagents; PyBOP®, PyAOP, TPTDP, BroP, PyBroP, DEPBT, and ethyl(hydroxyimino )cyanoacetate
This kit requires EvoluChem™ reaction block (HCK1006-01-002) included in the EvoluChem™ Starter Kit (HCK1006-01-001)
The typical protocol is performed at 0.1 mol/l concentration reaction condition using a solution of combined acid and amine.
Protocol at 100 μl volume reaction condition
- Prepare
required volume of a solution of combined amine 0.1 mol/L and acid substrate 0.1 mol/L in the desired solvent. For example, 700 μl solution for 6 reaction conditions (100 μl extra to compensate potential evaporation). - Add 2 equivalents of base (Et3N or DIPEA) to the solution see table
- Using a clean and dry syringe, add 100 μl of
solution to each reaction vial containing coupling reagent. - Optional: Add 10 μl of the solution of ethyl (
hydroxyimino ) cyanoacetate to each reaction vial. (prepared by adding 70 μl of DMF in the reagent vial) - Stir the reaction vials in the reaction block at
desired temperature for 3 to 12 hours. Do not heat vials attemperature higher than 120°C or higher than 5°C above the boiling point of the used solvent. - Remove the vial caps using a
decapper . - Prepare
analytical sample for each reaction condition with 5 μl sample diluted into 200 μl in either DMSO or water/acetonitrile 50/50.Analyze resulting analytical samples by LC/MS.
